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NOTE:
Academic Credits - The number of academic credits for each course is the number of credits earned in one semester with successful completion of that course.
Prerequisite and Corequisite - “Prerequisite” means that the listed course[s] must be successfully completed with the indicated grade. A “corequisite” may be taken in the same semester.
Course #
Course Description
Credits
BTN 101
Introduction to Biotechnology -- Prerequisite: BIO 121. This course provides a general introduction to the field of biotechnology. Research trends and methodologies currently exploited within the field are discussed along with the impact these endeavors may have on the quality of life. This course will survey the history, current and projected state of biotechnology as it relates to life science research, medicine, and agriculture. Laboratory will allow students to work with selected techniques in this field. 3 lecture hours; 2 lab hours.
4
   
BTN 103 Safety and Regulatory Compliance for Biotechnology -- Prerequisite: BIO 105 or BIO 121 or CHM 101 or CHM 105 or CHM 111. Overview of laboratory safety procedures and precautions, biosafety, radiation safety, compliance standards of regulatory agencies. Emphasis will be placed on understanding the regulatory environment of pharmaceutical, diagnostic and agricultural research and manufacturing. Students will be introduced to the concepts of current good laboratory practices [cGLP], current good manufacturing practices [cGMP], standard operating procedures [SOP] and validation as they apply to industries involved in biotechnology. 3 lecture hours. 3
   
BTN 201 Cell Culture and Cellular Processes -- Prerequisites: BTN 101, CHM 106. The student will be introduced to major biochemical pathways, cellular structure and function at a molecular level. Topics to be considered include the structure and function of the cell membrane, cytoskeleton and various organelles. Cellular respiration will be discussed. Protein synthesis, processing and export will be examined. Those processes involved in cell division will also be investigated and related to cancer. The laboratory will center upon techniques involving animal, plant, fungi and bacterial cell cultures. Students will be taught how to isolate, culture and preserve prokaryotic organisms, how to maintain and preserve eukaryotic cell cultures, and how to procure cell cultures from ATCC and other repositories. 2 lecture hours; 4 lab hours. 4
   
BTN 211 Analytic Methods in Biotechnology I -- Prerequisite: CHM 106. Students will become familiar with the theory and application of many analytical methods currently utilized in the field of biotechnology. These methods will include: ELISA and immunoaffinity techniques, methods for determining enzymatic activity, spectrophotometric methods, chromatographic methods, electrophoresis, light and electron microscopy. When feasible, techniques will be practiced in the laboratory setting. Methods utilizing radioactive isotopes will be discussed. Considerable emphasis will be placed on proper methods for data recording, analysis and presentation. 2 lecture hours; 2 lab hours.
3
   
BTN 212 Analytic Methods in Biotechnology II -- Prerequisite: BTN 211. Students will continue their study of the theory and application of many analytical methods currently utilized in the field of biotechnology. These methods will include: ELISA and immunoaffinity techniques, methods for determining enzymatic activity, spectrophotometric methods, chromatographic methods, electrophoresis, and light and electron microscopy. When feasible, techniques will be practiced in the laboratory setting. Methods utilizing radioactive isotopes will be discussed. Considerable emphasis will be placed on proper methods for data recording, analysis and presentation. 2 lecture hours; 2 lab hours. 3
   
BTN 217 Biotechnology Manufacturing Processes -- Prerequisite: CHT 211 or CHM 204. Student will be instructed in assembly, troubleshooting and standard operating procedures of manufacturing equipment associated with biotechnology including: pumps, valves, piping, tanks, mixing, material flow, pressure vessels, cryo-technology, freeze-drying, fill/label equipment, filter, homogenizers, automated inspections, etc. Students will be introduced to the regulatory environment facing most biotechnology endeavors including those mandated by FDA, USDA, OSHA, etc. 3 lecture hours; 2 lab hours. 4
   
BTN 227 Genetic Engineering and DNA Analysis -- Prerequisites: BTN 201, BTN 211. Students will become familiar with the theory and practice of common molecular biology techniques. Methods for manipulation of DNA and RNA that will be explored include nucleic acid extraction and electrophoresis, plasmid and phage construction, construction and screening of cDNA libraries and applications of PCR. Utilization of Internet-based genomic and proteomic databases will be emphasized. 2 lecture hours; 4 lab hours.
4
   
BTN 231 Fermentation -- Prerequisites: BTN 201, BTN 211. Students will be introduced to fermentation processes used for commercial purposes and the operation of small- and large-scale fermentors. Methods used to harvest product from fermentors and the regulatory requirements associated with commercial fermentation will also be explored. 2 lecture hours; 4 lab hours.
4
   
BTN 233 Protein Analysis and Purification -- Prerequisites: BTN 201, BTN 211. Students will be taught the theoretical basis of many commonly used protein purification methods [column chromatography, HPLC, affinity chromatography, immunoprecipitation, ultracentrifugation, etc.]. Students will be taught to determine which method is most applicable in various situations and why that method should be utilized. When possible, students will be given an opportunity to perform these techniques in the laboratory. 2 lecture hours; 4 lab hours. 4
   
BTN 280 Co-op/Internship -- Prerequisite: Program chair approval. Provides students with the opportunity to work at a job site that is specifically related to their career objectives. Provides on-the-job experience while earning credit toward an associate degree. Contact hours vary. 1-6
   
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